ELISpot Path: AdV5 (hexon) Human IFN-γ

ELISpot Path: AdV5 (hexon) Human IFN-γ

3420-2AST-P3-1

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A straightforward setup for functional analysis of a specific scientific question.

Components

Detection mAb7-B6-1, ALP
SubstrateBCIP/NBT-plus
PlatePre-coated ELISpot plate, strip (mAb 1-D1K)
Positive controlAnti-CD3 mAb (CD3-2)
Peptide poolAdV5 (hexon), human

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISpot Path kit is intended for the enumeration of cells secreting human IFN-γ in response to Adenovirus 5 hexon peptides using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Peptide pool included in the kit
The Adenovirus 5 hexon peptide pool contains 94 peptides derived from the hAdV5 hexon protein. It consists of both defined and scanning peptides. The scanning peptides are 15-mers overlapping with 11 amino acids. Together, these peptides cover immunodominant regions of the hAdV5 hexon protein that share sequence homology to human adenovirus 26 and chimpanzee adenovirus Y25. The mean purity of the synthetic peptides is 80%.

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Product details

ProductELISpot Path: AdV5 (hexon) Human IFN-γ
ApplicationELISpot
AnalyteIFN-γ
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human IFN-γ.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies, enzyme conjugate, and substrate at 4-8 °C. Peptide pools should be stored at -20 °C or below. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.
Human PBMC (250,000 cells/well) were incubated overnight in the absence or presence of the Adenovirus 5 hexon peptide (Mabtech) and the number of cells secreting IFN-γ was analyzed by ELISpot.
Datasheet/Protocol
SDS

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IFN-γ

Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69
Cell typeT cell, Tc, Th1, NK cell
Gene ID3458
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FluoroSpot Path: AdV5 (hexon) Human IFN-γ/IL-2